AKT3 (phospho-Ser472) antibody

• Catalog Number: orb6790
• Category: Tools
• Description: AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rabbit, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human AKT3 around the phosphorylation site of Ser472 (QF(p-S)YS)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IF=1:100-500, IHC-F=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Purity: Purified by Protein A
• Conjugation: Unconjugated
• MW: 56 kDa
• Target: phospho-AKT3 (Ser472)
• UniProt ID: P31749
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Akt3; AKT3 kinase; AKT3_HUMAN; DKFZp434N0250; PKB
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Yu-Qian Mao 1, Thiago V Seraphim 2, Yimei Wan 1, Ruikai Wu 1, Etienne Coyaud 3, Muhammad Bin Munim 1, Antonio Mollica 1, Estelle Laurent 3, Mohan Babu 4, Vito Mennella 5, Brian Raught 6, Walid A Houry DPCD is a regulator of R2TP in ciliogenesis initiation through Akt signaling Cell Rep, 43(2), 113713 (2024)

PubMed: 38306274

Applications

WB

Reactivity

Human

Western blot analysis of mouse lung lysate using AKT3 (phospho-Ser472) antibody

Western blot analysis of mouse brain lysate using AKT3 (phospho-Ser472) antibody

Western blot analysis of mouse lung lysate using AKT3 (phospho-Ser472) antibody

Immunohistochemical analysis of Mouse brain using AKT3 (phospho-Ser472) antibody

Immunohistochemical analysis of Rat brain using AKT3 (phospho-Ser472) antibody

Immunofluoroscence analysis of human lung carcinoma using AKT3 (phospho-Ser472) antibody