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Catalog Number | orb1260888 |
---|---|
Category | Antibodies |
Description | AKT2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant protein of human Akt2 |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 52kDa |
Target | AKT2 |
UniProt ID | P31751 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | rac protein kinase beta, Murine thymoma viral (v-a Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of 293 cells, using Akt2 antibody (orb1260888). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human breast using AKT2 antibody (orb1260888) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using AKT2 antibody (orb1260888) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using AKT2 antibody (orb1260888) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of C6 cells using AKT2 antibody (orb1260888) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using AKT2 antibody (orb1260888) at dilution of 1:100. Blue: DAPI for nuclear staining.
IF, IHC-P, WB | |
Bovine, Canine, Gallus, Porcine, Rabbit, Sheep |
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
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