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Catalog Number | orb1274477 |
---|---|
Category | Antibodies |
Description | AKT2 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | [8G3B6] |
Tested applications | ChIP, ICC, IF, IHC, IP, WB |
Reactivity | Human, Monkey, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant protein of human AKT2 |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:100ICC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:20 - 1:50ChIP: 1:20 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 60kDa |
Target | AKT2 |
UniProt ID | P31751 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | rac protein kinase beta, Murine thymoma viral (v-a Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:100ICC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:20 - 1:50ChIP: 1:20 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts from normal (control) and AKT2 knockout (KO) 293T cells, using AKT2 antibody (orb1274477) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
IF, IHC-P, WB | |
Bovine, Canine, Gallus, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
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