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Catalog Number | orb546987 |
---|---|
Category | Antibodies |
Description | AKT1 Monoclonal Antibody |
Species/Host | Rabbit |
Clonality | Monoclonal |
Clone Number | EBG-1 |
Tested applications | FC, ICC, IF, IHC, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthesized peptide derived from human AKT1 Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473. |
Concentration | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
Dilution range | WB 1:500-1:2000IHC 1:50-1:200ICC/IF 1:50-1:200IP 1:50FC 1:50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 56 kDa |
UniProt ID | P31749 |
Storage | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
WB analysis of AKT1 expression in MCF-7 Cell.
WB analysis of NOTCH1 expression in (1) HepG2 Cell; (2) A549 Cell; (3) HeLa Cell; (4) Raji Cell; (5) MCF-7 Cell; (6) SiHa Cell; (7) 293T Cell.
WB analysis of NOTCH1 expression in (1) Rat liver Tissue; (2) Rat brain Tissue; (3) Rat C6 Cell; (4) Rat RH35 Cell; (5) Mouse liver Tissue; (6) Mouse brain Tissue; (7) Mouse Neuro-2a Cell; (8) Mouse HEPA1-6 Cell.
Immunofluorescent analysis of HeLa cells, using AKT1 Antibody.
Immunohistochemical analysis of paraffin-embedded human colon, using AKT1 Antibody.
ELISA, FC, ICC, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
DOT, ELISA, FC, IHC, IP, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Biotin |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
IF, IH, IP, WB | |
Hamster, Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
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