AKT antibody

• Catalog Number: orb1474633
• Category: Antibodies
• Description: Rabbit monoclonal antibody to AKT
• Species/Host: Rabbit
• Clonality: Monoclonal
• Tested applications: IF, IH, IP, WB
• Reactivity: Hamster, Human, Mouse, Rat
• Immunogen: Recombinant protein of human AKT. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100), IP (1/10 - 1/50)
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Conjugation: Unconjugated
• Target: AKT1; AKT2; AKT3
• Entrez: 11652, 23797, 11651, 208, 207, 10000, 25233, 29414, 24185
• UniProt ID: P31751, Q60823, Q9Y243, Q63484, Q9WUA6, P47196, P31750, P31749, P47197
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Alternative names: AKT1; PKB; RAC; RAC-alpha serine/threonine-protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of AKT expression in Hela (A), Jurkat (B), rat brain (C), CHO-K1 (D) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 55 kD)

Immunohistochemical analysis of AKT staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of AKT staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.