AKT pT308 antibody (Biotin)

• Catalog Number: orb344542
• Category: Antibodies
• Description: AKT pT308 antibody (Biotin)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 18F3.H11
• Tested applications: DOT, ELISA, FC, IHC, IP, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: IgG1
• Immunogen: Anti-AKT pT308 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000, FC: User Optimized, IHC: 20 µg/ml, IP: User Optimized, WB: 1:500 - 1:3,000
• Form/Appearance: Lyophilized
• Purity: Anti-AKT pT308 was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at T308. A BLAST analysis was used to suggest cross-reactivity with AKT pT308 from most vertebrate species sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 will likely occur. Cancer, Cell Signaling, Neuroscience, Signal Transduction research.
• Conjugation: Biotin
• UniProt ID: P31749
• NCBI: 62241011
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-AKT pT308 Biotin conjugated Antibody, B
• Note: For research use only
• Application notes: Biotin Conjugated Anti-AKT pT308 is tested for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pT308 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required.
• Expiration Date: 12 months from date of receipt.

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of using AKT pT308 antibody (Biotin)

Immunohistochemical staining of Mouse Anti-AKT phospho T308 Biotin Conjugated at 20X (left) and 40X (right) Tissue: prostate Fixation using AKT pT308 antibody (Biotin)

Immunohistochemical staining of Mouse Anti-AKT phospho T308 Biotin Conjugated

Western blot analysis of GST tagged AKT1 active recombinant protein (Lane1). GST tagged AKT1 un-active recombinant protein (Lane2) using AKT pT308 antibody (Biotin)

Western blot analysis of GST tagged AKT1 active recombinant protein (Lane1), GST tagged AKT1 un-active recombinant protein (Lane2) using AKT pT308 antibody (Biotin)