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Catalog Number | orb1294322 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to AKT (Phospho-ser473) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | Human, Mouse, Rabbit |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide corresponding to residues surrounding Ser473 of human AKT |
Dilution range | Western Blot 1:500- 1:1000 Immunofluorescence 1:200-1:400 |
Conjugation | Unconjugated |
Target | Phospho-AKT (Ser473) |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
All lanes: Anti-AKT (pan) antibody at 1/500 dilution, Lysates/proteins at 60 μg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294322 overnight at 4°C.
ELISA, ICC, IF, IHC-P, WB | |
Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IHC-P, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
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