Akt1 (phospho-Ser473) antibody

• Catalog Number: orb526646
• Category: Tools
• Description: Akt1 (phospho-Ser473) antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, ICC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Akt1 around the phosphorylation site of Ser473 (QF(p-S)YS)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:50-200, ICC=1:100, Flow-Cyt=2ug/Test, IHC-F=1:50-200, IF=1:50-200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 56 kDa
• Target: Phospho-Akt1 (Ser473)
• UniProt ID: P31749
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Akt(Phospho-Ser473); AKT (phospho-S473); AKT (phos
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry of U-937.Primary Antibody (green line): Rabbit Anti-Phospho-Akt1(Ser473)antibody; Dilution:2ug/Test.

ICC analysis of NIH/3T3 cell using Phospho-Akt1(Ser473) monoclonal Antibody at 1:50 dilution;DAPI (blue) was used to stain the cell nuclei.

IHC-P analysis of mouse lung using Phospho-Akt1 Ser473 Monoclonal Antibody at 1:50 dilution.

Western blot:Lane 1:Human PC-3 cell;Primary:Anti-Phospho-Akt1(Ser473) at 1:500 dilution.

Western blot:Lane 1:NIH/3T3 treated with PDGF whole cell;Lane 2:Untreated NIH/3T3 whole cell;Primary:Anti-Phospho-Akt1(Ser473) at 1:500 dilution.