AKR1B1 Antibody

• Catalog Number: orb1264639
• Category: Antibodies
• Description: AKR1B1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This AKR1B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-135 amino acids from the Central region of human AKR1B1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 36 kDa
• Target: AKR1B1
• UniProt ID: P15121
• NCBI: P15121
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Aldose reductase, AR, Aldehyde reductase, Aldo-ket
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:8000 dilution Lane 1: A431 whole cell lysate Lane 2: A549 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of anti-AKR1B1 Pab in Jurkat cell line lysates (35 ug/lane)

Western blot analysis of AKR1B1 using rabbit polyclonal AKR1B1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AKR1B1 gene.

Confocal immunofluorescent analysis of AKR1B1 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AKR1B1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.