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AIF1/Iba1 Rabbit Polyclonal Antibody (BF488)
Catalog Number: orb1582554
| Catalog Number | orb1582554 |
|---|---|
| Category | Antibodies |
| Description | AIF1/Iba1 Rabbit Polyclonal Antibody (BF488) |
| Target | AIF1 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | BF488 |
| Reactivity | Human, Mouse, Rat |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Immunogen | KLH conjugated synthetic peptide derived from human Iba1 (51-147/147aa) |
| Tested applications | IF |
| Dilution range | IF=1:100-500 |
| Antibody Type | Primary Antibody |
| Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Alternative names | AIF 1; AIF1; AIF1 protein; IBa1; iba-1; IBa 1; all Read more... |
| Note | For research use only |
Blank control: U937. Primary Antibody (green line): Rabbit Anti-AIF1/Iba1 antibody (orb1582554), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
