Adipophilin Antibody / Perilipin 2 / ADRP / PLIN2

• Catalog Number: orb639593
• Category: Antibodies
• Description: Recognizes a protein of 48kDa, which is identified as Adipophilin. It belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ADFP/1365
• Tested applications: ELISA, FACS, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A human recombinant partial protein (amino acids 249-376) was used as the immunogen for this Adipophilin antibody.
• Antibody Type: Primary Antibody
• Dilution range: ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells in 0.1ml,Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Adipophilin antibody is available for research use only.
• UniProt ID: Q99541
• Storage: Store the Adipophilin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Flow cytometry testing of permeabilized human PMBC with Adipophilin antibody (clone ADFP/1365); Red=isotype control, Blue= Adipophilin antibody.

Western blot testing of a recombinant partial protein (immunogen, left) and human Jurkat cell lysate (right) with Adipophilin antibody (clone ADFP/1365). Predicted/observed molecular weight ~48 kDa.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Adipophilin antibody (clone ADFP/1365). These results demonstrate the foremost specificity of the ADFP/1365 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Adipophilin antibody (clone ADFP/1365) as confirmation of integrity and purity.