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Catalog Number | orb2635822 |
---|---|
Category | Antibodies |
Description | Recognizes a protein of 48kDa, which is identified as Adipophilin. It belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. |
Clonality | Recombinant |
Species/Host | Mouse |
Isotype | Mouse IgG2b, kappa |
Conjugation | Unconjugated |
Reactivity | Human |
Immunogen | A recombinant fragment (within amino acids 249-376) of human Adipophilin protein was used as the immunogen for the Adipose differentiation-related protein antibody. |
UniProt ID | Q99541 |
Tested applications | IHC-P |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml |
Application notes | Optimal dilution of the Adipose differentiation-related protein antibody should be determined by the researcher. |
Antibody Type | Primary Antibody |
Clone Number | rADFP/9321 |
Formula | 1 mg/ml in 1X PBS; BSA free, sodium azide free |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Hazard Information | This Adipose differentiation-related protein antibody is available for research use only. |
Note | For research use only |
IHC staining of FFPE human adrenal gland tissue with Adipose differentiation-related protein antibody (clone rADFP/9321). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Adipose differentiation-related protein antibody (clone rADFP/9321) as confirmation of integrity and purity.
ELISA, FACS, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
ELISA, IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
ELISA, IHC-P | |
Human | |
Mouse | |
Recombinant | |
Unconjugated |