Acidic Cytokeratin Antibody

• Catalog Number: orb385927
• Category: Antibodies
• Description: There are two types of cytokeratins/keratins/CKs: the acidic type I cytokeratins and the basic or neutral type II cytokeratins. The subsets of cytokeratins which an epithelial cell expresses depends mainly on the type of epithelium, the moment in the course of terminal differentiation and the stage of development. Thus this specific keratin fingerprint allows the classification of all epithelia upon their keratin expression profile. Furthermore this applies also to the malignant counterparts of the epithelia (carcinomas), as the keratin profile tends to remain constant when an epithelium undergoes malignant transformation. The main clinical implication is that the study of the keratin profile by immunohistochemistry techniques is a tool of immense value widely used for tumor diagnosis and characterization in surgical pathology. [Wiki]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ACCK1-1
• Tested applications: IHC-P
• Reactivity: Human, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: An amino acid sequence common to acidic/type I keratins was used as the immunogen for the Acidic Cytokeratin antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Acidic Cytokeratin antibody is available for research use only.
• UniProt ID: Q7Z794
• Storage: Store the Acidic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the Acidic Cytokeratin antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.3. This antibody will detect the following cytokeratins (CK): CK10: 56kDa; CK14: 50kDa; CK15: 50kDa; CK16: 48kDa; CK19: 40kDa.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human tonsil using Acidic Cytokeratin antibody

Immunohistochemical staining of Rat stomach using Acidic Cytokeratin antibody