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Catalog Number | orb1146986 |
---|---|
Category | Proteins |
Description | Insulin-like growth factor 1 (IGF-1) is also known as somatomedin C, IGF1A, IGFI, sulfation factor, and is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF-1, mecasermin is used for the treatment of growth failure. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 has a molecular weight of 7649 daltons. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. IGF-1 binds to at least two cell surface receptors: the Insulin-like growth factor 1 receptor, abbreviated as "IGF1R", and the insulin receptor. The IGF-1 receptor seems to be the "physiologic" receptor - it binds IGF-1 at significantly higher affinity than the IGF-1 that is bound to the insulin receptor. Like the insulin receptor, the IGF-1 receptor is a receptor tyrosine kinase - meaning it signals by causing the addition of a phosphate molecule on particular tyrosines. Its primary action is mediated by binding to its specific receptor IGF1R, present on many cell types in many tissues. Binding to the IGF1R, a receptor tyrosine kinase, initiates intracellular signaling; IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and proliferation, and a potent inhibitor of programmed cell death. Insulin-like growth factor 1 has been shown to bind and interact with all the IGF-1 Binding Proteins (IGFBPs), of which there are six (IGFBP1-6). Specific references are provided for interactions with IGFBP3, IGFBP4 and IGFBP7. |
Reactivity | Human |
Tag | N-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 9.5 kDa |
Target | IGF-I |
Source | Human IGF-I, His Tag, premium grade (orb1146986) is expressed from human 293 cells (HEK293). It contains AA Gly 49 - Ala 118 (Accession # P05019-1). |
Expression System | HEK293 |
Biological Activity | Biotinylated Human IGF-I R, His, immobilized on CM5 Chip can bind Human IGF-I, His Tag, premium grade with an affinity constant of 33.9 nM as determined in a SPR assay (Biacore 8K) (QC tested). |
Expression Region | Gly 49 - Ala 118 |
Endotoxins | 0.1 EU per μg |
NCBI | NP_001104755.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | IGF-I,IGF1A,somatomedin C,MGF Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 9.5 kDa. The protein migrates as 12 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Biotinylated Human IGF-I R, His, Avitag immobilized on CM5 Chip can bind Human IGF-I, His Tag, premium grade (Cat. No. orb1146986) with an affinity constant of 33.9 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Unconjugated | |
95% | |
27.0 kDa | |
Human IGFBP-7, His Tag (orb348791) is expressed from human 293 cells (HEK293). It contains AA Asp 30 - Leu 282 (Accession # NP_001544). |
Unconjugated | |
90% | |
52.6 kDa | |
Human IGFBP-7, Fc Tag (orb1146981) is expressed from human 293 cells (HEK293). It contains AA Asp 30 - Leu 282 (Accession # Q16270-1). |
Unconjugated | |
95% | |
129.3 kDa | |
Human IGF-I R, Fc Tag (orb1496214) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 932 (Accession # P08069-1). |
Unconjugated | |
90% | |
104.8 kDa | |
Human IGF-I R, His Tag (orb511230) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 932 (Accession # P08069-1). |
Unconjugated | |
90% | |
33.3 kDa | |
Human IGFBP-2 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 36 - Gln325 (Accession # P18065). |
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