Human IGFBP-7 Protein

• Catalog Number: orb348791
• Category: Proteins
• Description: Insulin-like growth factor-binding protein 7 (IGFBP7) is also known as IGFBP-rP1, MAC25 protein, PGI2-stimulating factor, prostacyclin-stimulating factor and tumor-derived adhesion factor, which contains one Ig-like C2-type (immunoglobulin-like) domain, one IGFBP N-terminal domain and one Kazal-like domain. The major function of IGFBP7 is the regulation of availability of insulin-like growth factors (IGFs) in tissue as well as in modulating IGF binding to its receptors. IGFBP7 binds to IGF with high affinity except for IGF-I and IGF-II.IGFBP7 also stimulates cell adhesion. Furthermore, IGFBP7 is implicated in some cancers.
• Reactivity: Human
• Tag: N-6×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 27.0 kDa
• Target: IGFBP-7
• Source: Human IGFBP-7, His Tag (orb348791) is expressed from human 293 cells (HEK293). It contains AA Asp 30 - Leu 282 (Accession # NP_001544).
• Expression System: HEK293
• Biological Activity: Human IGFBP-7, His Tagimmobilized on CM5 Chip can bind Human C1q R1, His Tag with an affinity constant of 16.7 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
• Expression Region: Asp 30 - Leu 282
• Endotoxins: 1.0 EU per μg
• NCBI: NP_001544
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: IBP7,IGFBP7
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 27.0 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human IGFBP-7 protein

Human IGFBP-7, His Tag (Cat. No. orb348791) immobilized on CM5 Chip can bind Human C1q R1, His Tag (Cat. No. orb257231) with an affinity constant of 16.7 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).