Human IGF-I R / CD221 Protein

• Catalog Number: orb511230
• Category: Proteins
• Description: The Insulin-like Growth Factor 1 Receptor (IGF1) is also known as CD221, JTK13. and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is make up of two alpha subunits and two beta subunits ,the Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain.The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 104.8 kDa
• Target: IGF-I R
• Source: Human IGF-I R, His Tag (orb511230) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 932 (Accession # P08069-1).
• Expression System: HEK293
• Biological Activity: Human IGF-I R, His Tagimmobilized on CM5 Chip can bind Biotinylated Human IGF-I, His, (Cat. No.IG1-H82Q6) with an affinity constant of 68 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
• Expression Region: Glu 31 - Asn 932
• Endotoxins: 1.0 EU per μg
• NCBI: NP_000866.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: IGF1R,IGFR,JTK13,CD221,MGC142170,MGC142172,MGC1821
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin convertase cleavage site, 737-RKRR-740, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.0 kDa and 23.8 kDa respectively. The protein migrates as 35-45 kDa (partial β chain), 110-120 kDa (α chain) and 130 kDa (α chain & partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Human IGF-I R, His Tag (Cat. No. orb511230) immobilized on CM5 Chip can bind Biotinylated Human IGF-I, His, Avitag with an affinity constant of 68 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).