ZO-1 Rabbit Polyclonal Antibody

SKU: orb11587

Description

ZO-1 Rabbit Polyclonal Antibody is an unconjugated antibody that specifically recognizes ZO-1. Generated against a KLH conjugated synthetic peptide derived from human ZO-1. The peptide is available as orb374898. The suitable applications for this antibody are ELISA, FACS, IHC-P.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, FACS, IHC-P
• Dilution Range: P-ELISA: 1:500-1000 (based on 0.5 mg/ml)

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ZO-1. Please contact us for the exact immunogen sequence. The peptide is available as orb374898.
• Target: ZO-1
• Molecular Weight: 191 kDa
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Concentration: 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti-DKFZp686M05161 antibody, anti-MGC133289 antibody, anti-Tight junction protein 1 antibody, anti-Tight junction protein ZO1 antibody, anti-TJP1 antibody, anti-ZO-1 antibody, anti-Zona occludens 1 protein antibody, anti-Zonula occludens 1 protein antibody

Quick Database Links

Gene Symbol

ZO-1

UniProt

Q07157, P39447

NCBI Gene ID

7082

RefSeq:003248, 31, 003257

Woolf, Eric C. et al. The Ketogenic Diet Alters the Hypoxic Response and Affects Expression of Proteins Associated with Angiogenesis, Invasive Potential and Vascular Permeability in a Mouse Glioma Model PLoS One, 10, e0130357 (2015)

PubMed: 26083629

Applications

WB

Reactivity

Mouse

Zhao, Yuan et al. Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets Int J Mol Sci, 12, 8502-8512 (2011)

PubMed: 22272087

Applications

IHC

Reactivity

Porcine

Anna Arczewska-Wlosek,Sylwester Swiatkiewicz,Ewa Tomaszewska,Siemowit Muszynski,Piotr Dobrowolski and Damian Jozefiak Effects of Anticoccidial Vaccination and Taraxacum officinale Extract on the Growth Performance, Biochemical Parameters, Immunity, and Intestinal Morphology of Eimeria-Challenged Chickens MDPI, 13(9), 1927 (2023)

PubMed: 37763330

Applications

IHC

Reactivity

Gallus

Lili Liu , Xiaoyi Gong , Xinyu Zhang , Dawei Zhang , Ying Tang ,Jiantao Liu , Yajie Li , Disheng Pan Resveratrol Alleviates Heat-Stress-Induced Impairment of the Jejunal Mucosa Through TLR4/MAPK Signaling Pathway in Black-Boned Chicken Poultry Science, 103242 (2023)

PubMed: 37980746

Applications

IHC

Reactivity

Gallus