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Catalog Number | orb1247325 |
---|---|
Category | Antibodies |
Description | XRCC6 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: YKTEGDEEAEEEQ-C |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 70kDa band observed in lysates of cell lines A431, HeLa and HepG2 (calculated MW of 69.8kDa according to NP_001460.1). In transfected HEK293 transiently expressing KU70 a band of approx. 70kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.1-0.3ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | XRCC6 |
UniProt ID | P12956 |
NCBI | NP_001460.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | XRCC6, KU70, X-ray repair complementing defective Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 70kDa band observed in lysates of cell lines A431, HeLa and HepG2 (calculated MW of 69.8kDa according to NP_001460.1). In transfected HEK293 transiently expressing KU70 a band of approx. 70kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.1-0.3ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1247325 (0.1 ug/ml) staining of HeLa cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing KU70 and probed with orb1247325 (mock transfection in first lane).
IF, IHC, IP, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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