XRCC6 Antibody

• Catalog Number: orb1256461
• Category: Antibodies
• Description: XRCC6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 408-609 of human KU70 (NP_001460.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 70kDa
• Target: XRCC6
• UniProt ID: P12956
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: G22P1, ML8, KU70, TLAA, CTC75, CTCBF, G22P1
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using KU70 antibody (orb1256461) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunohistochemistry of paraffin-embedded rat brain using KU70 antibody (orb1256461) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using KU70 antibody (orb1256461) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human uterus using KU70 antibody (orb1256461) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A549 cells using KU70 antibody (orb1256461). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 100 ug extracts of SW480 cells using 3 ug KU70 antibody (orb1256461). Western blot was performed from the immunoprecipitate using KU70 antibody (orb1256461) at a dilution of 1:1000.