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Catalog Number | orb546300 |
---|---|
Category | Antibodies |
Description | WNT4 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human WNT4 recombinant protein (Position: K100-E274). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Direct ELISA, 0.1-0.5μg/ml |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 39 kDa |
UniProt ID | P56705 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Protein Wnt-4; WNT4; UNQ426; PRO864 Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of WNT4 using anti-WNT4 antibody.Lane 1:human HEK293 cell;2:human PC-3 cell;3:human U-87MG cell;4:human HeLa cell.
IHC analysis of WNT4 using anti-WNT4 antibody.WNT4 was detected in paraffin-embedded section of human liver cancer tissues.
IHC analysis of WNT4 using anti-WNT4 antibody.WNT4 was detected in paraffin-embedded section of human renal cancer tissues.
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