WNT4 Antibody

• Catalog Number: orb1271026
• Category: Antibodies
• Description: WNT4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit Ig
• Immunogen: This WNT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-239 amino acids from the Central region of human WNT4.
• Concentration: batch dependent
• Dilution range: For FACS starting dilution is: 1:25For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 39 kDa
• Target: WNT4
• UniProt ID: P56705
• NCBI: P56705
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Protein Wnt-4, WNT4
• Note: For research use only
• Application notes: For FACS starting dilution is: 1:25For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Overlay histogram showing MCF-7 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Western Blot at 1:2000 dilution Lane 1: MDA-MB-231 whole cell lysate Lane 2: mouse bladder lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: human testis lysate Lane 3: mouse bladder lysate Lane 4: rat lung lysate Lysates/proteins at 20 ug per lane.

Formalin-fixed and paraffin-embedded human bladder carcinoma reacted with WNT4 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.