Vinculin Antibody

• Catalog Number: orb1294416
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Vinculin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the center region.
• Dilution range: Western Blot 1:2000 - 1:4000 Immunofluorescence 1:200 – 1:500 Immunohistochemistry (Paraffin) 1:200 – 1:300
• Conjugation: Unconjugated
• Target: Vinculin
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin embedded mouse testis labeling Vinculin with orb1294416 at 1:300.

Western blot analysis using Anti-Vinculin antibody at 1:3000 dilution, lysates/proteins at 30 μg per lane.

Western blot analysis using Anti-Vinculin antibody at 1:3000 dilution, lysates/proteins at 30 μg per lane.

Immunofluorescence:cells were stained with anti-Vinculin antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.