Vinculin Antibody

• Catalog Number: orb1526455
• Category: Antibodies
• Description: Vinculin Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 1016 and 1066
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 5 - 15 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Vinculin
• UniProt ID: P18206
• NCBI: NP_003364.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: HEL114; vinculin; MVCL; MV; metavinculin; epididym
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Vinculin by western blot. Samples: Whole cell lysate (50 µg) from HeLa, K-562, PC-3, HEK293T, and OVCAR-4 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Vinculin antibody (orb1526455) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of mouse Vinculin by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Vinculin antibody (orb1526455) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Vinculin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from PC-3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Vinculin antibody (orb1526455) used for IP at 6 µg per reaction.

Detection of human Vinculin by immunohistochemistry.Sample: FFPE section of human testis. Antibody: Affinity purified rabbit anti- Vinculin (Cat. No. orb1526455) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human Vinculin by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- Vinculin (Cat. No. orb1526455) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB