VCL Antibody

• Catalog Number: orb1240274
• Category: Antibodies
• Description: VCL Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Predicted Reactivity: Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-Vinculin antibody (orb1240274) was raised against a peptide corresponding to 23 amino acids near the center of human Vinculin. <
• Concentration: 1 mg/mL
• Dilution range: WB: 0.5-2 μg/mL; ICC: 5 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 124kDObserved: 124kD
• Target: VCL
• UniProt ID: P18206
• NCBI: NP_003364
• Storage: Vinculin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
• Buffer/Preservatives: Vinculin antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Vinculin Antibody: MV, MVCL, CMD1W, CMH15, HEL114,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: Vinculin orb1240276 (2 µg/mL), Vinculin orb1240285 (1 µg/mL), Vinculin orb1240274 (1 µg/mL), and beta-actin orb1240312 (1.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: Vinculin orb1240274 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in PC3 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: Vinculin orb1240274 (Lane 1: 0.5 µg/mL and Lane 2: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry Validation of Vinculin in Jurkat Cells. Immunocytochemical analysis of Jurkat cells using anti-Vinculin antibody (orb1240274) at 5 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of Vinculin in Jurkat Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Jurkat cells labeling Vinculin with orb1240274 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).