Vimentin antibody

• Catalog Number: orb669777
• Category: Antibodies
• Description: Rabbit monoclonal antibody to Vimentin
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: RV205
• Tested applications: ICC, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Conjugation: Unconjugated
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• UniProt ID: P08670
• Source: This product is a recombinant rabbit anti vimentin monoclonal antibody produced in mammalian HEK293 cells. Clone RV205 is a reformatted antibody (species switch), produced by cloning the antibody sequence of the mouse anti vimentin hybridoma RV202, which was derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.
• Storage: Storage: The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Indirect immunofluorescence staining of normal human dermal fibroblasts in tissue culture with orb669777 (diluted 1:100), showing the specific cytoskeletal pattern of vimentin intermediate filaments.

Indirect immunofluorescence staining of human kidney tissue section with orb669777 (diluted 1:1000), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, podocytes, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment.

Indirect immunofluorescence staining of human kidney tissue section with orb669777 (diluted 1:1000), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, podocytes, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment.

Immunostaining of human paraffin embedded tissue section of placenta with orb669777 (diluted 1:200), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment.

Immunostaining of human paraffin embedded tissue section of testis with orb669777 (diluted 1:200), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, endothelial cells in blood vessels and Sertoli cells.

Immunostaining of human paraffin embedded tissue sections of human colon with orb669777 (diluted 1:200), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment.

Immunostaining of human paraffin embedded tissue sections of human kidney with orb669777 (diluted 1:200), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, and podocytes. As expected, no reactivity is seen in the epithelial cell compartment.

Immunostaining of human paraffin embedded tissue sections of human small intestine with orb669777 (diluted 1:200), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue. As expected, no reactivity is seen in the epithelial cell compartment.

Immunostaining of human paraffin embedded tissue sections of human spleen with orb669777 (diluted 1:200), showing the specific pattern of vimentin in the mesenchymal cell types.

Non-reduced and reduced SDS-PAGE of orb669777 showing its purity.