Vimentin Antibody

• Catalog Number: orb1294389
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Vimentin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Dilution range: Western Blot 1:2000-1:5000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:200–1:300 Immunoprecipitation: Reported in the literature, not verified in house.
• Conjugation: Unconjugated
• Target: Vimentin
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Vimentin with orb1294389 at 1:300.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Vimentin with orb1294389 at 1:300.

Lysates at 30 μg per lane.

Lysates at 30 μg per lane.

Immunofluorescence:cells were stained with anti-Vimentin antibody (red) at 1:500 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling Vimentin with orb1294389 at 1:300.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling Vimentin with orb1294389 at 1:300.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling Vimentin with orb1294389 at 1:300.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling Vimentin with orb1294389 at 1:300.

Lysates/proteins at 30 μg per lane.