Vimentin Antibody

• Catalog Number: orb251542
• Category: Antibodies
• Description: Vimentin Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Vimentin (435-466aa DTHSKRTLLIKTVETRDGQVINETSQHHDDLE), identical to the related mouse and rat sequences.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 53652 MW
• UniProt ID: P08670
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Vimentin;VIM;
• Note: For research use only
• Application notes: WB: The detection limit for Vimentin is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB:Lane 1:human placenta tissue; 2:human U-87MG cell; 3:human U20S cell;4:human PC-3 cell;5:human HeLa cell;6:human THP-1 cell;7:human A549 cell;8:human MCF-7 cell;9:rat ovary tissue;10:rat lung tissue;11:mouse lung tissue;12:mouse NIH/3T3 cell.

IF analysis of Vimentin using anti-Vimentin antibody. Vimentin was detected in a paraffin-embedded section of mouse brain tissue.

IF analysis of Vimentin using anti-Vimentin antibody. Vimentin was detected in a paraffin-embedded section of rat brain tissue.

IHC analysis of Vimentin using anti-Vimentin antibody. Vimentin was detected in a paraffin-embedded section of mouse cardiac muscle tissue.

IHC analysis of Vimentin using anti-Vimentin antibody. Vimentin was detected in a paraffin-embedded section of human mammary cancer tissue.

IHC analysis of Vimentin using anti-Vimentin antibody. Vimentin was detected in a paraffin-embedded section of rat cardiac muscle tissue.

IHC analysis of Vimentin using anti-Vimentin antibody. Vimentin was detected in a paraffin-embedded section of human placenta tissue.

IHC analysis of Vimentin using anti-Vimentin antibody. Vimentin was detected in a paraffin-embedded section of human lung adenocarcinoma tissue.

IHC analysis of Vimentin using anti-Vimentin antibody. Vimentin was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue.

IHC analysis of Vimentin using anti-Vimentin antibody. Vimentin was detected in a paraffin-embedded section of human invasive urothelial carcinoma tissue.

IHC analysis of Vimentin using anti-Vimentin antibody. Vimentin was detected in a paraffin-embedded section of human liver cancer tissue.

IHC analysis of Vimentin using anti-Vimentin antibody. Vimentin was detected in a paraffin-embedded section of human tonsil tissue.