USP2 antibody

• Catalog Number: orb33724
• Category: Antibodies
• Description: Purified rabbit polyclonal antibody.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB4417
• Tested applications: IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: IHC-P: 1:50-100, WB: 1:2000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 68072
• Target: USP2
• UniProt ID: O75604
• NCBI: NP_004196.4, NP_001230688.1, NP_741994.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti-Ubiquitin carboxyl-terminal hydrolase 2 antib
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Davis, Mindy I. et al. Small Molecule Inhibition of the Ubiquitin-specific Protease USP2 Accelerates cyclin D1 Degradation and Leads to Cell Cycle Arrest in Colorectal Cancer and Mantle Cell Lymphoma Models J Biol Chem, 291, 24628-24640 (2016)

PubMed: 27681596

Immunohistochemical staining of paraffin embedded human testis tissue using USP2 antibody (primary antibody dilution at: 1:50-100)

Western blot analysis of in K562 cell line lysates (35ug/lane)using USP2 antibody (antibody dilution at 1:1000)

Western blot analysis of 293 cell lysateusing USP2 antibody (primary antibody dilution at: 1:1000)

Western blot analysis of MCF-7 cell lineusing USP2 antibody (dilution of primary antibody at 1:500-1:1000)