UBE2D1 Antibody

• Catalog Number: orb1264992
• Category: Antibodies
• Description: UBE2D1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Bovine, Mouse, Rat
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This UBE2D1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the C-terminal region of human UBE2D1.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:100For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 17 kDa
• Target: UBE2D1
• UniProt ID: P51668
• NCBI: P51668
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Ubiquitin-conjugating enzyme E2 D1, Stimulator of
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:100For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded H. liver section using UBE2D1 Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from A431, HepG2 cell line, human skeletal muscle and mouse thymus tissue lysate (from left to right), using hUBE2D1-I126 at 1:1000 at each lane.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.