Cart summary

You have no items in your shopping cart.

    UBC9 antibody

    Catalog Number: orb1474419

    DispatchUsually dispatched within 5-10 working days
    $ 413.00
    Catalog Numberorb1474419
    CategoryAntibodies
    DescriptionRabbit monoclonal antibody to UBC9
    Species/HostRabbit
    ClonalityMonoclonal
    Tested applicationsIF, IH, IP, WB
    ReactivityHuman, Mouse, Rat
    ImmunogenA synthetic peptide of human UBE2I/UBC9
    Dilution rangeWB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100), IP (1/10 - 1/50)
    Form/AppearanceLiquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
    ConjugationUnconjugated
    TargetUBE2I
    Entrez102641751, 25573, 7329, 22196
    UniProt IDP63281, P63279, P63280
    SourceRabbit
    StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
    Buffer/PreservativesLiquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
    Alternative namesUBC9; UBCE9; SUMO-conjugating enzyme UBC9; SUMO-pr
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    UBC9 antibody

    Western blot analysis of UBC9 expression in Hela (A), A549 (B), HL60 (C), NIH3T3 (D), PC12 (E), MEF (F) whole cell lysates. (Predicted band size: 18 kD; Observed band size: 18 kD)

    UBC9 antibody

    Immunohistochemical analysis of UBC9 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.132). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    UBC9 antibody

    Immunofluorescent analysis of UBC9 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

    • UBE2I UBC9 Antibody [orb443231]

      FC,  ICC,  IF,  IHC,  IHC-Fr,  WB

      Human, Mouse, Rat

      Rabbit

      Polyclonal

      Unconjugated

      10 μg, 100 μg
    • UBE2I/UBC9 Antibody [orb570417]

      ELISA,  FC,  ICC,  IF,  IHC,  WB

      Human, Mouse, Rat

      Rabbit

      Polyclonal

      Unconjugated

      10 μg, 100 μg
    • UBE2I Antibody [orb97386]

      FC,  ICC,  IHC,  WB

      Human, Monkey

      Mouse

      Monoclonal

      Unconjugated

      100 μl
    • SUMO2 Antibody [orb749787]

      IHC-P

      Human, Rat

      Mouse

      Monoclonal

      Unconjugated

      20 μg, 100 μg
    • SUMO2/3 Antibody [orb248545]

      FACS,  IF,  IHC-P,  WB

      Human

      Mouse

      Monoclonal

      Unconjugated

      100 μg, 20 μg
    Submit a review

    Filter by Rating

      • Star
      • Star
      • Star
      • Star
      • Star
      • 5 stars
      • Star
      • Star
      • Star
      • Star
      • Star
      • 4 stars
      • Star
      • Star
      • Star
      • Star
      • Star
      • 3 stars
      • Star
      • Star
      • Star
      • Star
      • Star
      • 2 stars
      • Star
      • Star
      • Star
      • Star
      • Star
      • 1 stars