UBE2I/UBC9 Antibody

• Catalog Number: orb570417
• Category: Antibodies
• Description: UBE2I/UBC9 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human UBE2I/UBC9 recombinant protein (Position: K65-K146 ).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 18 kDa
• UniProt ID: P63279
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: SUMO-conjugating enzyme UBC9; RING-type E3 SUMO tr
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis using anti-UBE2I/UBC9 antibody.Lane 1:A549 cell, Lane 2:Hepg2 cell, Lane 3:PC-3 cell, Lane 4:Hek293 cell, Lane 5:rat lung tissue, Lane 6:mouse ANA-1 cell.

Flow Cytometry analysis of A431 cells using anti-UBE2I/UBC9 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.

IF analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody.

IHC analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody.

IHC analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody.

IHC analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody.

IHC analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody.

IHC analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody.

IHC analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody.

IHC analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody.