UBASH3A antibody

• Catalog Number: orb19426
• Category: Antibodies
• Description: Goat polyclonal antibody to UBASH3A
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Canine, Human
• Dilution range: ELISA: 1:64000, WB: 0.1 μg/ml, IHC-P: 2.5 μg/ml
• Conjugation: Unconjugated
• MW: 74.1; 69.8
• Target: STS2 / TULA (internal)
• Entrez: 53347
• Protein Sequence: QQCSTPDGKYYRPD
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti TULA antibody, anti STS2 CBL-interacting prot
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Spleen shows cytoplasm staining of T-cells eccentrically gathered around an artery. Recommended concentration, 2.5ug/ml.WB: Preliminary experiments gave an approx 48kDa band in lysates of B-cell line Daudi after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 74.1kDa according to NP_061834.1 and 69.8kDa according to NP_001001895.1. The 48kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb19426 (2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.