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Catalog Number | orb1252454 |
---|---|
Category | Antibodies |
Description | TYRP1 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | TYRP1/807 |
Tested applications | FC, IF, IHC-P |
Reactivity | Human, Mouse |
Isotype | IgG2a, kappa |
Immunogen | Recombinant full-length human protein was used as the immunogen for the TYRP1 antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Flow Cytometry: 1-2 ug/million cells in 0.1mlImmunofluorescence: 3-6 ug/mlImmunohistochemistry (FFPE): 3-6 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the TYRP1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | TYRP1 |
UniProt ID | P17643 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | 6-dihydroxyindole-2-carboxylic acid oxidase, DHICA Read more... |
Note | For research use only |
Application notes | Flow Cytometry: 1-2 ug/million cells in 0.1mlImmunofluorescence: 3-6 ug/mlImmunohistochemistry (FFPE): 3-6 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the TYRP1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed, paraffin-embedded human melanoma stained with TYRP1 antibody (TYRP1/807)
ICC, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Animal, Bovine, Canine, Goat, Guinea pig, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish | |
Canine, Equine, Goat, Guinea pig, Human, Mouse, Rat, Sheep, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FA, FACS, Kinetics | |
Human | |
Monoclonal | |
Unconjugated |
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