TUBB3 Antibody

• Catalog Number: orb1672015
• Category: Antibodies
• Description: TUBB3 Antibody
• Clonality: Recombinant
• Clone Number: S11B
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG lambda
• Immunogen: Human MBP (microtubule-binding protein).
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: TUBB3
• UniProt ID: Q13509
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TUBB
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluoresence staining of fixed HeLa cells with anti-Beta-tubulin antibody S11B. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton stained with the chimeric r version of S11B (orb1672015) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672015- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor® 488 secondary antibody.

Flow-cytometry using the anti-Beta Tubulin S11B. MCF-7 (A) and HeLa (B) cells were stained with unimmunized r antibody (black line) or the rmeric version of S11B (orb1672015 - blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.

Western Blot using anti-Beta-Tubulin antibody S11B. HeLa (A)- A431 (B)- HEK293 (C) and MCF-7 (D) cell lysate samples (35 ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of S11B (orb1672015) at 0.01 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Beta-Tubulin is ~54kDa. orb1672015 successfully detected human Beta-Tubulin in HeLa- A431- HEK293 and MCF-7 cell lysate samples.

Immunohistochemical staining of human cerebral cortex tissue using anti-Beta Tubulin antibody. S11B Anti-Beta Tubulin staining of paraffin embedded human cerebral cortex tissue using the rmeric version of S11B (orb1672015). Antigen retreival was acheived by microwaving in citrate buffer (pH6)- followed by blocking with protein block serum-free buffer. Primary antibody incubation with orb1672015 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-r HRP secondary antibody for 20 mins followed by Ddiaminobenzidine- and counter-staining with haemotoxylin. Staining of neuronal cell bodies and their processes may be observed. Recommended concentration- 2-4 ug/ml.