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Catalog Number | orb1258462 |
---|---|
Category | Antibodies |
Description | TUBB3 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | [5D5D2] |
Tested applications | WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant protein of human TUBB3. |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 55kDa |
Target | TUBB3 |
UniProt ID | Q13509 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | beta-4, CDCBM, CFEOM3A, class III beta-tubulin, OT Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using TUBB3 antibody (orb1258462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
FC, ICC, IHC-P, WB | |
Canine, Human, Mouse, Porcine, Rat | |
Monoclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC-P, WB | |
Bovine, Gallus, Monkey, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Bovine, Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Canine, Gallus, Hamster, Human, Insect, Monkey, Mouse, Rabbit, Rat, Sheep, Yeast | |
Mouse | |
Monoclonal | |
Unconjugated |
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