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Catalog Number | orb18424 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to TRIM28 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IF, IHC |
Reactivity | Human, Mouse |
Dilution range | ELISA: 1:2000, IHC-P: 2-4 μg/ml |
Conjugation | Unconjugated |
MW | 88.5 |
Target | KAP1 / TRIM28 |
Entrez | 10155 |
Protein Sequence | SSQELSGGPGDGP |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti TRIM28 antibody, anti KAP1 antibody, anti TF1 Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: This product has been successfully used on Human gastric cancer surgical specimens: Yokoe et al, Ann Surg Oncol. 2009 Nov 7. [Epub ahead of print]. (PMID: 19898899). Recommended concentration: 5ug/ml.WB: Approx 100-120kDa band seen in HepG2 lysate. Recommended for use at 0.2-1 μg/ml (Predicted MW approx 92kDa according to NP_005753).Experiment Notes: Immunofluorescence: Successfully used on mouse embryo at the late zygote stage, 5ug/ml (personal communication). |
Expiration Date | 12 months from date of receipt. |
orb18424 staining (0.5ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemical analysis of formalin-fixed and paraffin embedded Human Breast using KAP1 antibody
Immunohistochemical staining of Human Breast using TRIM28 antibody
Western blot analysis of HepG2 lysate using TRIM28 antibody
FC, ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IHC, IP, SW-Size, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
FC, ICC, IHC, IP, SW-Size, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
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