KAP1/TRIM28 Antibody

• Catalog Number: orb334567
• Category: Antibodies
• Description: KAP1/TRIM28 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human KAP1 recombinant protein (Position: A699-P835). Human KAP1 shares 94.9% amino acid (aa) sequence identity with both mouse and rat KAP1.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 88550 MW
• UniProt ID: Q13263
• Sensitivity: < 20pg/ml
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Transcription intermediary factor 1-beta;TIF1-beta
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-KAP1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of KAP1 using anti-KAP1 antibody.Lane 1:human HeLa cell;2:human PC-3 cell;3:human 293T cell;4:human A549 cell;5:rat PC-12 cell;6:mouse spleen tissue;7:mouse NIH/3T3 cell.

IF analysis of KAP1 using anti-KAP1 antibody. KAP1 was detected in a paraffin-embedded section of human ovarian cancer tissue.

IHC analysis of KAP1 using anti-KAP1 antibody. KAP1 was detected in a paraffin-embedded section of human mammary cancer tissue.

IHC analysis of KAP1 using anti-KAP1 antibody. KAP1 was detected in a paraffin-embedded section of rat intestine tissue.

IHC analysis of KAP1 using anti-KAP1 antibody. KAP1 was detected in a paraffin-embedded section of mouse intestine tissue.

IHC analysis of KAP1 using anti-KAP1 antibody. KAP1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue.

IHC analysis of KAP1 using anti-KAP1 antibody. KAP1 was detected in a paraffin-embedded section of human gastric carcinoma tissue.

IHC analysis of KAP1 using anti-KAP1 antibody. KAP1 was detected in a paraffin-embedded section of human liver cancer tissue.

IHC analysis of KAP1 using anti-KAP1 antibody. KAP1 was detected in a paraffin-embedded section of human ovarian cancer tissue.