KAP-1, Phospho (S824) Antibody

• Catalog Number: orb1519970
• Category: Antibodies
• Description: KAP-1, Phospho (S824) Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, ICC, IHC, IP, SW-Size, WB
• Reactivity: Human
• Immunogen: surrounding serine 824
• Concentration: 100 µg/ml
• Dilution range: WB - 1:1000; IP - 20 µl/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC-IF - 1:100 - 1:500. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: KAP-1, Phospho (S824)
• UniProt ID: Q13263
• NCBI: NP_005753.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: KRAB [Kruppel-associated box domain]-associated pr
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (25 µg) from HEK293T cells treated with 100 µM etoposide (+) or mock treated (-) prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (orb1519970) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-KAP1 recombinant monoclonal antibody [BL-248-2G6].

Detection of mouse Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (50 µg) from NIH3T3 cells treated with 100 µM etoposide (+) or mock treated (-) prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (orb1519970) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-KAP1 recombinant monoclonal antibody [BL-248-2G6].

Detection of human Phospho KAP-1 (S824) in FFPE prostate carcinoma by IHC. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (orb1519970). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Detection of human phospho KAP-1 (shaded) in etoposide treated HEK293T cells (right) and untreated HEK293T cells (left) by flow cytometry. Antibody: Rabbit anti-phospho KAP-1 recombinant monoclonal [BL-246-7B5] (orb1519970) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG.

Detection of human Phospho KAP-1 (S824) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer that were treated with 100 µM etoposide (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (orb1519970) used for IP at 20 µl/mg lysate.

Detection of human Phospho KAP-1 (S824) in FFPE etoposide treated HeLa cells by ICC. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (orb1519970). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Detection of human Phospho KAP-1 (S824) by immunocytochemistry. Samples: Formaldehyde-fixed asynchronous HeLa cells grown in chambered microscope slides and treated with etoposide (right) or untreated (left). Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (orb1519970) used at of 1:100. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG. Counterstain: Phalloidin conjugated Alexa Fluor® 488 (green).

Detection of human Phospho KAP-1 (S824) by Simple Western™. Samples: Whole cell lysate (0.4 mg/mL) from HEK293T cells treated with 100 µM etoposide prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (orb1519970) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.