Transcription factor SOX-2 SOX2 Antibody

• Catalog Number: orb178730
• Category: Antibodies
• Description: Transcription factor SOX-2 SOX2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SOX2 (171-183aa YSMMQDQLGYPQH), different from the related mouse and rat sequence by one amino acid.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 34310 MW
• UniProt ID: P48431
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Transcription factor SOX-2;SOX2;
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of SOX2 using anti-SOX2 antibody.Lane 1:human U251 cell; 2:human 293T cell.

IHC analysis of SOX2 using anti-SOX2 antibody. SOX2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue.

IHC analysis of SOX2 using anti-SOX2 antibody. SOX2 was detected in a paraffin-embedded section of mouse brain tissue.

IHC analysis of SOX2 using anti-SOX2 antibody. SOX2 was detected in a paraffin-embedded section of rat brain tissue.