TP53 Antibody

• Catalog Number: orb1252137
• Category: Antibodies
• Description: TP53 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PAb 1801
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Human p53 beta-galactosidase fusion protein was used as the immunogen for this antibody. Its epitope maps near the N-terminal end (aa 32-79) of p53.
• Concentration: 0.2 mg/mL
• Dilution range: Western blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cellsImmunofluorescence: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Variations in protocols, secondaries and substrates may require the p53 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: TP53
• UniProt ID: P04637
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Cellular tumor antigen p53, Antigen NY-CO-13, Phos
• Note: For research use only
• Application notes: Western blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cellsImmunofluorescence: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Variations in protocols, secondaries and substrates may require the p53 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Analysis of HuProt(TM) microarray containing more than 19000 full-length human proteins using p53 antibody (clone PAb 1801). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC testing of FFPE human colon carcinoma with p53 antibody (clone Pab 1801). Required HIER: boil tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human A431 cell lysate with p53 antibody (clone Pab 1801). Expected molecular weight ~53 kDa.