TP53 Antibody

• Catalog Number: orb1257343
• Category: Antibodies
• Description: TP53 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 218-390 of mouse p53 (NP_035770.2).
• Concentration: batch dependent
• Dilution range: WB: 1:3000 - 1:6000IHC: 1:50 - 1:200IP: 1:20 - 1:50ChIP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 53kDa
• Target: TP53
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: TRP53, bbl, bfy, bhy, p44, p53, Tp53
• Note: For research use only
• Application notes: WB: 1:3000 - 1:6000IHC: 1:50 - 1:200IP: 1:20 - 1:50ChIP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using p53 antibody (orb1257343) at 1:6000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Immunohistochemistry of paraffin-embedded rat ovary using p53 antibody (orb1257343) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using p53 antibody (orb1257343) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using p53 antibody (orb1257343) at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 150 ug extracts of A549 cells using 3 ug p53 antibody (orb1257343). Western blot was performed from the immunoprecipitate using p53 antibody (orb1257343) at a dilution of 1:500.

Chromatin immunoprecipitation analysis of extracts of 293T cells, using p53 antibody (orb1257343) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.