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Catalog Number | orb1336382 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to p53 (TP53) |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | OTI3D5 |
Tested applications | FC, IF, IHC, WB |
Reactivity | Human |
Isotype | IgG1 |
Immunogen | Full length human recombinant protein of human TP53 (NP_000537) produced in HEK293T cell. |
Dilution range | WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100 |
Conjugation | Unconjugated |
MW | 43.5 kDa |
Target | TP53 |
UniProt ID | P04637 |
NCBI | NM_000546 |
Note | For research use only |
Application notes | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Expiration Date | 12 months from date of receipt. |
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53 (RC200003, Right lane) cDNA for 48 hrs and lysed.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TP53 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min)
Anti-TP53 mouse monoclonal antibody (TA502780) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TP53)
HEK293T cells transfected with either RC200003 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TP53 antibody (TA502780), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-TP53 antibody (TA502780), (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometric Analysis of Jurkat cells, using anti-TP53 antibody (TA502780), (Red), compared to a nonspecific negative control antibody, (Blue).
ELISA, ICC, IF, IHC-P, WB | |
Bovine, Canine, Porcine, Rabbit, Sheep, Xenopus | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ChIP, ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
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