TNFRSF8 Antibody

• Catalog Number: orb1671982
• Category: Antibodies
• Description: TNFRSF8 Antibody
• Clonality: Recombinant
• Clone Number: Ki-3
• Tested applications: Activation, ELISA, FC, IF, IHC
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: Human CD30.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: TNFRSF8
• UniProt ID: P28908
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TNFRSF8, Tumor necrosis factor receptor superfamil
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the anti-CD30 (Tumor necrosis factor receptor superfamily member 8) antibody Ki-3. Jurkat cells were stained with unimmunized r antibody (black line) or the rmeric version of Ki-3 (orb1671982 - blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using an anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.

Immunohistochemical staining of human tonsil tissue using anti-CD30 antibody. Ki-3 Anti-CD30 staining of paraffin embedded human tonsil tissue using the rmeric version of Ki-3 (orb1671982). Antigen retreival was acheived by microwaving in citrate buffer (pH6)- followed by blocking with protein block serum-free buffer. Primary antibody incubation with orb1671982 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-r HRP secondary antibody for 20 mins followed by Ddiaminobenzidine- and counter-staining with haemotoxylin. Staining of the cellular membrane of dividing cells may be observed. Recommended concentration- 2-4 ug/ml.