CD30 Antibody

• Catalog Number: orb1519861
• Category: Antibodies
• Description: CD30 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human
• Immunogen: residues 19-385 (ECD)
• Concentration: 250 µg/ml
• Dilution range: WB - 1:1000; IP - 5 µl/1 mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CD30
• UniProt ID: P28908
• NCBI: NP_001234.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: D1S166E; tumor necrosis factor receptor superfamil
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human CD30 by western blot. Samples: Whole cell lysate (50 µg) from MJ, HEK293T, SR, GaMG, and K-562 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD30 recombinant monoclonal antibody (orb1519861) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human CD30 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-CD30 recombinant monoclonal antibody (orb1519861). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human CD30 (shaded) in MJ[G11] cells by flow cytometry. Antibody: Rabbit anti-CD30 recombinant monoclonal (orb1519861) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG.

Detection of human CD30 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD30 recombinant monoclonal antibody (orb1519861) used for IP at 20 µl/mg lysate.

Detection of human CD30 by immunocytochemistry. Sample: FFPE section of SR cells. Antibody: Rabbit anti-CD30 recombinant monoclonal antibody (orb1519861). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human CD30 (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-CD30 recombinant monoclonal antibody (orb1519861) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).