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    TNFRSF8 Antibody

    Catalog Number: orb1252801

    DispatchUsually dispatched within 5-10 working days
    $ 1,003.00
    Catalog Numberorb1252801
    CategoryAntibodies
    DescriptionTNFRSF8 Antibody
    Species/HostMouse
    ClonalityMonoclonal
    Clone NumberCD30/412
    Tested applicationsELISA, FC, IHC
    ReactivityHuman
    IsotypeIgG2b, kappa
    ImmunogenHuman CD30 recombinant protein was used as the immungen for this antibody.
    Concentration0.2 mg/mL
    Dilution rangeELISA: order BSA-free format for coatingFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD30 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
    Form/AppearanceLiquid
    ConjugationUnconjugated
    TargetTNFRSF8
    UniProt IDP28908
    StorageAliquot and Store at 2-8°C. Avoid freez-thaw cycles.
    Buffer/PreservativesPBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
    Alternative namesTumor necrosis factor receptor superfamily member
    Read more...
    NoteFor research use only
    Application notesELISA: order BSA-free format for coatingFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD30 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
    Expiration Date12 months from date of receipt.
    TNFRSF8 Antibody

    IHC testing of Hodgkin's lymphoma (20X) stained with CD30 antibody (CD30/412).

    TNFRSF8 Antibody

    IHC testing of Hodgkin's lymphoma (20X) stained with CD30 antibody (CD30/412).

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