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    TNFRSF1B antibody

    Catalog Number: orb214685

    DispatchUsually dispatched within 5-10 working days
    $ 337.00
    Catalog Numberorb214685
    CategoryAntibodies
    DescriptionRabbit polyclonal antibody to TNFRSF1B
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIF, IH, WB
    ReactivityHuman, Mouse, Rat
    ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD120b. The exact sequence is proprietary.
    Dilution rangeWB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500, IP: 1-10-1-100
    Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    ConjugationUnconjugated
    TargetTNFRSF1B
    Entrez156767, 7133, 21938
    UniProt IDP20333, P25119, Q80WY6
    SourceRabbit
    StorageShipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
    Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    Alternative namesTNFBR; TNFR2; Tumor necrosis factor receptor super
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    TNFRSF1B antibody

    Western blot analysis of CD120b expression in HEK293T (A), A375 (B), DLD (C), mouse brain (D), mouse heart (E), rat brain (F), rat heart (G) whole cell lysates. (Predicted band size: 48 kD; Observed band size: 65 kD)

    TNFRSF1B antibody

    Immunohistochemical analysis of CD120b staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    TNFRSF1B antibody

    Immunofluorescent analysis of CD120b staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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      FC,  ICC,  IHC-Fr,  IHC-P,  IP,  WB

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      Polyclonal

      Unconjugated

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