TIGIT antibody

• Catalog Number: orb613975
• Category: Antibodies
• Description: Mouse monoclonal antibody to TIGIT
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1B4
• Tested applications: Blocking, ELISA, FC
• Reactivity: Mouse
• Isotype: IgG1
• Immunogen: This antibody was raised by immunising TIGIT-/- mice with recombinant murine TIGIT tetramers.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: TIGIT
• UniProt ID: P86176
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: VSTM3; T cell Ig and ITIM domain; T cell immunorec
• Note: For research use only
• Application notes: The binding specificity of this antibody for TIGIT has been confirmed in ELISA analysis and by flow cytometry using P815 cells transfected with mouse TIGIT and activated mouse splenocytes (Dixon et al, 2018). This antibody also specifically stains activated primary wild-type T cells (Dixon et al, 2018). This antibody fully blocks CD155 binding to TIGIT, and acts as a TIGIT blocking antibody in vivo. Administration of this antibody increases experimental autoimmune encephalomyelitis (EAE) severity, and retards the growth of established MC38 colon carcinoma and GL261 glioblastoma (Dixon et al, 2018). This antibody synergises with anti-PD-1 blocking antibodies to achieve complete tumour regression in colon carcinoma models (Dixon et al, 2018). The original mouse IgG1 antibody does not deplete TIGIT-expressing CD4+ or CD8+ T cells in vivo, nor affect T cell proliferation in vitro (Dixon et al, 2018).
• Expiration Date: 12 months from date of receipt.