TIGIT Antibody

• Catalog Number: orb1240136
• Category: Antibodies
• Description: TIGIT Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3B5
• Tested applications: ELISA, FC, ICC, IF, IHC-P
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: TIGIT antibody was raised against the extracellular domain of human TIGIT
• Concentration: 1 mg/mL
• Dilution range: TIGIT antibody can be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 1 μg/mL. For flow cytometry at 1 μg/ml. For immunocytochemistry at 1 μg/mL. Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 26 kDa Observed: 47 kDa
• Target: TIGIT
• UniProt ID: Q495A1
• NCBI: NP_776160
• Storage: TIGIT antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: TIGIT Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Alternative names: TIGIT Antibody: T-cell immunoreceptor with Ig and
• Note: For research use only
• Application notes: TIGIT antibody can be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 1 μg/mL. For flow cytometry at 1 μg/ml. For immunocytochemistry at 1 μg/mL. Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry of TIGIT in over expressing HEK293 cells using TIGIT antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.

Immunofluorescence of TIGIT in over expressing HEK293 cells using TIGIT Antibody at 1 μg/ml. Green: TIGIT Antibody [3B5] (orb1240136) Blue: DAPI staining

Immunofluorescence of TIGIT in human stomach carcinoma tissue using TIGIT Antibody at 5 μg/ml. Green: TIGIT Antibody [3B5] (orb1240136) Blue: DAPI staining

Immunohistochemistry of TIGIT in human stomach carcinoma tissue using TIGIT Antibody and control mouse IgG (corner box) at 2 μg/ml.

Flow cytometry analysis of TIGIT over expressing HEK293 cells using TIGIT antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.

Titration curve analysis of TIGIT mAbs to detect recombinant TIGIT in ELISA with orb1240147, orb1240136, orb1240137, orb1240140, orb1240146 and orb1240115 antibodies at decreasing concentrations.