TIGIT Antibody

• Catalog Number: orb1821587
• Category: Antibodies
• Description: TIGIT Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4A5
• Tested applications: Blocking, ELISA, FC
• Reactivity: Human
• Isotype: IgG
• Immunogen: The original antibody was generated by immunizing 9 week old BALB/c mice with recombinant human TIGIT extracellular region Fc fusion protein in complete Freund's adjuvant.
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: TIGIT
• UniProt ID: Q495A1
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: VSTM3; VSIG9; WUCAM; T-cell immunoreceptor with Ig
• Note: For research use only
• Application notes: The binding characterization of this antibody towards human TIGIT was done using ELISA and flow cytometry. This antibody can bind human TIGIT extracellular domain Fc fusion protein in an ELISA and is also capable of recognizing TIGIT expressed on HEK293 cells by flow cytometry. The original IgG1 version of this antibody binds human TIGIT with a binding affinity of Kd=3.732 X 10^(-10) M. This antibody was also reported to successfully block the binding of TIGIT to its ligand CD155. This antibody is capable of binding NK cells in vitro. The anti-cancer activity of this antibody was tested on ovarian cancer cell HO8910, human non-small cell lung cancer cell NCl-H358 and human non-small cell lung cancer cell NCl-H1650 (CN109384846).
• Expiration Date: 12 months from date of receipt.