TIA1 antibody

• Catalog Number: orb19498
• Category: Antibodies
• Description: Goat polyclonal antibody to TIA1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 3.75 μg/ml
• Conjugation: Unconjugated
• MW: 41.8; 43.0
• Target: TIA1
• Entrez: 7072
• Protein Sequence: PKSTYESNTKQ
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti TIA1 antibody, anti TIA1 cytotoxic granule-as
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 40kDa band observed in lysates of cell line Jurkat (calculated MW of 41.8kDa according to NP_071320.1). Recommended concentration: 0.1-0.3 μg/ml. An additional band at 80kDa and a very strong 26kDa is also observed. These bands were successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates under reduced conditions Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb19498 (0.1ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19498 (3.75µg/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunofluorescence analysis of HeLa cells using TIA1 antibody